SYBR GREEN I Nucleic Acid Gel Stain CAS:163795-75-3

SYBR Green I iganda renucleic acid gel rakajairika mukuyedza kwe molecular biology. Idhayi re fluorescent rinosunga zvakananga kumamorekuru eDNA neRNA ane tambo mbiri, zvichiita kuti aonekwe pasi pechiedza che ultraviolet. SYBR Green I inonyanya kubatwa uye inogona kushandiswa kuona nekuverenga DNA kana RNA mumagels e agarose kana polyacrylamide. Inowanzoshandiswa paDNA gel electrophoresis, PCR analysis, uye mamwe mashandisirwo e molecular biology.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

DNA gel electrophoresis: SYBR Green I vara rinowedzerwa kuDNA gel mushure me electrophoresis, uye kana rakaiswa pachiedza cheUV, rinobatana neDNA bands, zvichiita kuti pave nechiedza chakajeka. Nzira iyi yekuisa mavara inobatsira kuona nekuongorora zvidimbu zveDNA zvakakamurwa nehukuru.

Quantitative PCR (qPCR): SYBR Green I inowanzo shandiswa muqPCR kutarisa kuwedzera kweDNA kana RNA sequences munguva chaiyo. Dhayi iyi inosunga kuDNA ine tambo mbiri inogadzirwa panguva yekuwedzera kwePCR, uye kuwedzera kwesimba re fluorescence kunoyerwa padanho rega rega rekuwedzera, zvichibvumira kuyerwa kwe template yekutanga ye nucleic acid.

RNA gel electrophoresis: SYBR Green I inogona kushandiswawo paRNA gel electrophoresis, zvichibvumira kuona nekuongorora zvidimbu zveRNA. Inobatsira pakuongorora kuvimbika kweRNA uye pakudzidza mazinga ekutaura kweRNA.

Kunyora uye kuona nucleic acid: SYBR Green I inogona kushandiswa kunyora nucleic acids ne fluorescence. Izvi zvinogona kuitwa nekushandisa dhayi panguva yekugadzira nucleic acid kana nekushandisa nzira dzekunyora mushure mekunyora. Nucleic acids dzakanyorwa dzinogona kuonekwa uye kuonekwa uchishandisa masisitimu e fluorescent imaging.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C32H37N4S
Kuyedza	99%
Chitarisiko	Mvura isina ruvara
Nhamba yeCAS	163795-75-3
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.