TRIS-Acetate CAS: 6850-28-8 Mutengo wemugadziri

TRIS-Acetate, ibuffer inowanzoshandiswa mukuyedza kwezvipenyu nemakemikari. Isanganiswa yeTris base neacetic acid, zvichikonzera mhinduro yakasimba yepH inoshandiswa kudzora nekuchengetedza huwandu hwepH hunodiwa kune akasiyana mashandisirwo. TRIS-Acetate inonyanya kubatsira muzvidzidzo zveDNA neRNA, sezvo ichipa nzvimbo yakakodzera yemabasa eenzyme, electrophoresis, uye gel electrophoresis. Inobatsira kuchengetedza kugadzikana uye kusimba kwe nucleic acids panguva dzakasiyana-siyana, dzakadai seDNA sequencing, polymerase chain reaction (PCR), uye agarose gel electrophoresis. Kuwedzera kune nucleic acid research, TRIS-Acetate inoshandiswawo mu protein isolation and purification procedures, membrane protein extraction, uye cell culture experiments. Kugona kwayo kuchinjika kunoita kuti ive chishandiso chakakosha munzvimbo dzakawanda dzesainzi research, ichivimbisa mamiriro akanakisa e biological reactions uye kuchengetedza kugadzikana kwema enzyme nemaprotein.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Tris-acetate (TRIS-Acetate) chinhu chinoshandiswa zvakanyanya mukuyedza kwezvipenyu nemakemikari. Chine musanganiswa wetris(hydroxymethyl)aminomethane (Tris) neacetic acid, iyo inoshanda se pH regulator uye stabilizer. pH yeTRIS-Acetate buffer inowanzo bva pa7.4 kusvika 8.4.
Mhedzisiro huru yeTRIS-Acetate ndeyekuchengetedza pH yakagadzikana, iyo inokosha kune akawanda maitiro ehupenyu uye emakemikari. Inoshanda senzira yekudzivirira nekuderedza chero shanduko dzakakosha mupH dzinogona kuitika nekuda kwekuwedzera maacid kana mabhesi panguva yekuedza.
TRIS-Acetate inowana mashandisirwo akasiyana-siyana mu molecular biology, biochemistry, uye biotechnology:
Electrophoresis yeDNA neRNA: TRIS-Acetate inowanzoshandiswa senzira yekudzivirira kupararira kwemasero mu electrophoresis yeagarose nepolyacrylamide gel. Inopa pH yakagadzikana panguva yekupatsanurwa kwezvidimbu zveDNA neRNA zvichienderana nehukuru hwazvo.
Kuongorora Mapuroteni: TRIS-Acetate buffers inoshandiswa pakugadzira mapuroteni emagetsi, akadai seSDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Inovimbisa kugadzikana uye kupatsanurwa kwemapuroteni panguva yekushanda.
Maitiro EmaEnzyme: Mabuffer eTRIS-Acetate anoshandiswa kakawanda mukuyedza maEnzyme nezvidzidzo. Anopa huwandu hwakanaka hwepH kune akasiyana-siyana maitiro emaEnzymatic uye anobatsira kuchengetedza mashandiro emaEnzyme.
Kukura Kwemasero Nenyama: TRIS-Acetate buffers inoshandiswa mukukura kwemasero kuchengetedza pH yakakodzera yekukura kwemasero uye kuwanda kwawo. Inobatsira kuchengetedza mamiriro emuviri anodiwa kuti masero arambe achirarama.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C6H15NO5
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	6850-28-8
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.