5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium munyu CAS:18656-96-7

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt, inozivikanwawo seX-glucuronide, ikemikari inoshandiswa zvakanyanya mukuyedza kwe molecular biology uye enzymology. I chromogenic substrate inowanzoshandiswa kuona mashandiro e enzyme beta-D-glucuronidase.

Musanganiswa uyu une boka re indole rakabatana ne glucuronic acid moiety kuburikidza nechisungo che glycosidic. Bromo ne chloro substituents zvinopa kugadzikana uye zvinowedzera kunyunguduka kwemusanganiswa uyu.

Kana ikaiswa muchimiro chemuenzaniso une beta-D-glucuronidase, enzyme iyi inocheka chisungo cheglycosidic, yoburitsa chikamu che indole. Izvi zvinoita kuti pave nekuchinja kweruvara rwebhuruu, zvichiita kuti zvive nyore kuona kana kuona mashandiro e enzyme.

Nekuda kwehunyanzvi hwayo hwe beta-D-glucuronidase, X-glucuronide inoshandiswa zvakanyanya muhunyanzvi hwe molecular biology hwakadai se bacterial and yeast transformation assays, reporter gene assays, uye histochemical staining. Inopa nzira iri nyore uye inonzwisisika yekuona kuvapo kana kuratidzwa kwe beta-D-glucuronidase uye inowanzo shandiswa pamwe chete nedzimwe genetic kana biochemical assays.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Mhedzisiro ye5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (X-glucuronide) kugona kwayo kugadzira shanduko yebhuruu kana ruvara kana paine enzyme beta-D-glucuronidase. Iyi enzyme reaction inoitika kana chisungo cheglycosidic pakati peboka reindole nechikamu cheglucuronic acid chikachekwa ne beta-D-glucuronidase.

Kushandiswa kweX-glucuronide kunonyanya kuitwa muongororo ye molecular biology uye enzyme. Inowanzoshandiswa se substrate yekuona mashandiro e beta-D-glucuronidase. Mamwe mashandisirwo chaiwo anosanganisira:

Kuongororwa kwemajini emutori wenhau: X-glucuronide inowanzo shandiswa sechikamu chekuongorora majini emutori wenhau uko beta-D-glucuronidase inoshandiswa seenzyme yemutori wenhau. Nekubatanidza nzvimbo yekukurudzira kana kunyora kutevedzana kwejini rinonakidza kune jini re beta-D-glucuronidase, vaongorori vanogona kuyera kuratidzwa kana kushanda kwejini nekutarisa kuumbwa kwebhuruu precipitate kana shanduko yeruvara kana X-glucuronide yawedzerwa.

Kuongororwa kwekushanduka kwebhakitiriya nembiriso: X-glucuronide inogona kushandiswa kuona kushanduka kunobudirira kwebhakitiriya kana mbiriso nevector ine gene re beta-D-glucuronidase. Masero akachinjwa anoratidza enzyme anogona hydrolyze X-glucuronide, zvichiita kuti ruvara ruonekwe kana kuti ruve rwakajeka.

Kudhirowa kweHistochemical: X-glucuronide inoshandiswa muhunyanzvi hwedhirowa yehistochemical kuona kuvapo nekupararira kwemaitiro e beta-D-glucuronidase mumatishu kana masero. Kudhirowa uku kunobvumira kuwanikwa uye kuzivikanwa kwemasero anoratidza enzyme.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C26H36BrClN2O7
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	18656-96-7
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.