8-Anilino-1-naphthalenesulfonic acid CAS:82-76-8

8-Anilino-1-naphthalenesulfonic acid, inozivikanwawo seANS, idhayi re fluorescent rinowanzoshandiswa muongororo ye biochemical uye biophysical. Iri muboka remakemikari e organic anozivikanwa se aniline-based sulfonic acids. ANS inozivikanwa nekukwanisa kwayo kubatana nenzvimbo dzinopisa mvura kana nzvimbo dzinopisa mvura mumapuroteni nedzimwe biomolecules.

ANS inopenya kana yakabatana nenzvimbo dzisingadiwi nemhepo, zvichiita kuti ive probe inobatsira pakudzidza shanduko dzemapuroteni, kudyidzana kwemapuroteni nemapuroteni, uye maitiro ekupeta/kuvhura mapuroteni. Kubatana kweANS nenzvimbo dzisingadiwi nemhepo kunotungamira kukuchinja kwebhuruu mu emission spectrum, iyo inogona kutariswa uchishandisa fluorescence spectroscopy.

ANS inowanzoshandiswa mumhando dzakasiyana dzekutsvaga, kusanganisira fluorescence polarization, fluorescence resonance energy transfer (FRET), uye thermal stability assays. Yakashandiswa kudzidza chimiro nekugadzikana kwemapuroteni, kudyidzana kwemapuroteni nema ligand, uye maitiro ekuunganidza mapuroteni.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Chiyero cheFluorescent: ANS inoshandiswa zvakanyanya sechiyero chefluorescent kudzidza shanduko dzemapuroteni, maitiro ekupeta/kuvhura mapuroteni, uye kudyidzana kwemapuroteni neligand. Chinosunga kunzvimbo dzinopisa kana nzvimbo dzinopisa mumapuroteni, zvichikonzera kubuda kwefluorescence kunogona kutariswa uchishandisa fluorescence spectroscopy.

Kuona Hydrophobicity: ANS inonzwa shanduko muhydrophobicity ye microenvironment. Inogona kushandiswa kuona uye kuyera surfactants, sipo, nezvimwe zvinhu zvinosvibisa hydro.

Kuongororwa kwekugadzikana kwemapuroteni: ANS inowanzo shandiswa mukuyedza kugadzikana kwemapuroteni nekupisa kuti ione kugadzikana kwemapuroteni. Nekutarisa kusimba kwefluorescence yemapuroteni ane ANS pasi pekupisa kwakasiyana, zvinokwanisika kuona hunhu hwekupisa uye kupisa kwekunyunguduka (Tm) kwepuroteni.

Zvidzidzo zvekuunganidza mapuroteni: ANS inogona kushandiswa kudzidza maitiro ekuunganidza mapuroteni, akadai sekuumbwa kweamyloid fibrils. Dhayi iyi inonzwa nzvimbo dzine hydrophobic mumapuroteni aggregates, zvichiita kuti ive chishandiso chakakosha chekutarisa kufambira mberi uye kinetics yekuunganidzwa.

Kutsanangurwa kwekubatana kwemapuroteni nemapuroteni: ANS yakashandiswa kuongorora kupindirana kwemapuroteni nemapuroteni. Kana mapuroteni achipindirana, panogona kuve nekuchinja kwekusagadzikana kwawo mumvura, izvo zvinogona kuongororwa uchishandisa ANS fluorescence.

Kuwanikwa kwemishonga uye kugadzirwa kwayo: ANS inogona kushandiswa pakuongorora vanhu vanogona kushandisa mishonga. Inogona kubatsira kuongorora kubatana uye kudyidzana kwemamorekuru madiki nemapuroteni anotarisirwa, zvichibvumira vaongorori kuona makemikari anogona kugadzirisa mashandiro emapuroteni.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C16H13NO3S
Kuyedza	99%
Chitarisiko	Upfu hwegirini yakasviba
Nhamba yeCAS	82-76-8
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.