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PNPG CAS:3150-24-1 Mutengo wemugadziri
PNPG, kana p-nitrophenyl β-D-glucopyranoside, idiki molecule substrate inowanzoshandiswa mu biochemical assays kuyera basa reglucosidase enzymes.Haina ruvara uye haina-fluorescent, asi pahydrolysis neglucosidase, inoshandurwa kuita p-nitrophenol, ine yero ruvara uye inoonekwa zviri nyore spectrophotometrically.
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ONPG CAS:369-07-3 Mutengo Wemugadziri
O-nitrophenyl-β-D-galactopyranoside (ONPG) is a synthetic substrate inoshandiswa mu biochemical uye molecular biology assays kuyera basa re enzyme β-galactosidase.Inowanzoshandiswa mukuedza kuona kuratidzwa kwejene mumasisitimu ebhakitiriya, akadai seEscherichia coli.ONPG isanganiswa isina ruvara inotsemurwa ne β-galactosidase, zvichikonzera kuburitswa kweyero compound, o-nitrophenol.Iyo yero ruvara inogadzirwa inogona kuyerwa spectrophotometrically, ichipa chiyero chisina kunanga chekuita kwe enzyme.Chiyero chinoshandisa ONPG chinowanzo kunzi ONPG assay uye inzira inoshandiswa zvakanyanya mukutsvagisa mamolecular biology kuongorora mazinga ekutaura kwemajini anodzorwa nelac. operon mumasero ebhakitiriya.
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Nitrotetrazolium Blue Chloride CAS:298-83-9
Nitrotetrazolium Bhuruu Chloride (NBT) chiratidzo che redox chinowanzo shandiswa mubiological uye biochemical assays.Iyo yakachenuruka yehupfu inoshanduka bhuruu kana yaderedzwa, zvichiita kuti ibatsire pakuona kuvepo kwemamwe ma enzymes uye metabolic chiitiko.
NBT inopindirana nema electron carriers uye ma enzymes akadai se dehydrogenases, ayo anobatanidzwa mumaitiro akasiyana-siyana emasero.Kana NBT ikaderedzwa nemaenzayimu aya, inoumba bhuruu formazan precipitate, inobvumira kuona kana kuona spectrophotometric.
Iyi reagent inowanzoshandiswa mukuedza senge NBT yekuderedza bvunzo, iyo inoshandiswa kuongorora kuputika kwekufema kwemasero ekudzivirira.Inogona zvakare kushandiswa kudzidza enzyme zviitiko uye nzira dzemetabolism mukutsvaga zvine chekuita neoxidative kusagadzikana, kushanda kwesero, uye kusiyanisa masero.
NBT yakawana mashandisirwo muzvikamu zvakasiyana, kusanganisira microbiology, immunology, uye cell biology.Inoshandiswa zvakasiyana-siyana, yakagadzikana, uye iri nyore kushandisa, zvichiita kuti ive sarudzo yakakurumbira kune akawanda mapuroteni ekuedza.
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Neocuproine CAS:484-11-7 Mutengo Wemugadziri
Neocuproine ndeye chelating agent iyo inowanzoshandiswa mumashandisirwo akasiyana-siyana, kusanganisira analytical chemistry uye pharmaceuticals.Iyo ine hukama hwakasimba hwecopper ions uye inoumba yakagadzikana yakaoma pamwe navo.Ichi chivakwa chinoita kuti neocuproine ibatsire pakuona uye kuverengera mhangura mumhinduro kana sampuli.Pamusoro pezvo, neocuproine yakadzidzwa kune yayo inogona kurapa maapplication, kunyanya mukurapa cancer uye neurodegenerative zvirwere.
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IPTG CAS:367-93-1 Mutengo wemugadziri
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a synthetic analogi ye lactose iyo inowanzoshandiswa mukutsvagisa molecular biology uye biotechnology application.IPTG inonyanya kushandiswa kukurudzira kutaura kwemajini muhurongwa hwemabhakitiriya, uko inoshanda senge molecular trigger kutanga kunyorwa kwemajini anotangwa.
Kana yawedzerwa kune yekukura svikiro, IPTG inotorwa nebhakitiriya uye inogona kusunga kune lac repressor protein, ichidzivirira kubva pakuvhara basa re lac operon.Iyo lac operon iboka remajini anobatanidzwa mu lactose metabolism, uye kana puroteni inodzvinyirira yabviswa, majini anoratidzirwa.
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HATU CAS:148893-10-1 Mutengo wemugadziri
HATU (1- [bis(dimethylamino) methylene] -1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) inowanzoshandiswa coupling reagent mu peptide synthesis uye organic chemistry.
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D-fucose CAS:3615-37-0 Mutengo wemugadziri
D-fucose inonzi monosaccharide, kunyanya shuga yekabhoni matanhatu, inova yeboka reshuga dziri nyore dzinonzi hexoses.Iyo isomeri yeglucose, inosiyana mukugadzirisa kweimwe hydroxyl boka.
D-fucose inowanikwa muzvisikwa zvakasiyana-siyana, kusanganisira mabhakitiriya, fungi, zvidyarwa, uye mhuka.Inotamba mabasa akakosha mune akati wandei maitiro ehupenyu, senge sero kusaina, sero adhesion, uye glycoprotein synthesis.Icho chikamu che glycolipids, glycoproteins, uye proteoglycans, iyo inobatanidzwa musero-kune-sero kutaurirana uye kuzivikanwa.
Muvanhu, D-fucose inobatanidzwawo mu biosynthesis yezvivako zvakakosha zveglycan, zvakadai seLewis antigens uye maantigen eboka reropa, izvo zvine zvazvinoreva mukuwedzerwa kuwedzerwa kweropa uye kubatwa kwechirwere.
D-fucose inogona kuwanikwa kubva kwakasiyana masosi, kusanganisira yegungwa, zvidyarwa, uye microbial fermentation.Inoshandiswa mukutsvagisa uye biomedical application, pamwe nekugadzira mimwe mishonga nemishonga yekurapa.
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DDT CAS:3483-12-3 Mutengo wemugadziri
DL-Dithiothreitol, inozivikanwawo seDTT, inhengo inodzikisa inowanzoshandiswa mukutsvakurudza kwebiochemical uye molecular biology.Imorekuru diki rine thiol (sarufa-ine) boka pamagumo ega ega.
DTT inowanzoshandiswa kuputsa zvisungo zve disulfide mumapuroteni, izvo zvinobatsira kuvhura kana kuti denature.Uku kudzikiswa kwezvisungo zvedisulfide kwakakosha mumatanho akasiyana emurabhoritari senge protein kucheneswa, gel electrophoresis, uye zvidzidzo zveprotein chimiro.DTT inogona zvakare kushandiswa kuchengetedza thiol mapoka uye kudzivirira oxidation panguva yekuedza maitiro.
DTT inowanzowedzerwa kumhinduro dzekuyedza muzvikamu zvidiki, uye kuita kwayo kunoenderana nekuvapo kweokisijeni.Zvakakosha kubata DTT zvakanyatsonaka sezvo inonzwa kumhepo, kupisa, uye unyoro, izvo zvinogona kuderedza kushanda kwayo.
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D-(+)-Galactose CAS:59-23-4 Mutengo wemugadziri
D-(+)-Galactose ishuga yemonosaccharide uye chikamu chinokosha chemaitiro akawanda ehupenyu.Ishuga inongoitika yega inowanikwa muzvokudya zvakawanda, zvakadai semichero, zvigadzirwa zvemukaka, nemiriwo.
Galactose inowanzo gadziriswa mumuviri kuburikidza nekutevedzana kwemaitiro enzymatic.Inobata basa rakakosha mukutaurirana kwesero, kugadzira simba, uye biosynthesis yemamorekuru akakosha senge glycolipids, glycoproteins, uye lactose.
Panyaya yemashandisirwo ayo, D-(+) -Galactose inowanzoshandiswa mune microbiology uye biotechnology seyakagadzika kabhoni mune tsika midhiya yekukura kwehutachiona hwakasiyana.Inoshandiswawo mukugadzirwa kweakasiyana bioactive komisheni, mishonga, uye zvigadzirwa zvekudya.Uyezve, inowanzoshandiswa sechirwere chekuongorora chirwere, kunyanya mukuedzwa kwekuongorora kushanda kwechiropa uye kuona zvirwere zvemajini zvine chekuita negalactose metabolism.
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beta-D-Galactose pentaacetate CAS:4163-60-4
Beta-D-Galactose pentaacetate ikemikari inotorwa kubva kugalactose, shuga yemonosaccharide.Inoumbwa neacetylating yega yega hydroxyl boka regalactose molecule ine mashanu acetyl mapoka.
Mushonga uyu unowanzo shandiswa sechinhu chinodzivirira chegalactose mune akasiyana makemikari maitiro uye maitiro ekugadzira.Iyo pentaacetate fomu inobatsira kudzikamisa galactose uye kudzivirira zvisingadiwe maitiro kana shanduko panguva yekuita.
Pamusoro pezvo, komisheni iyi inogona kushandiswa sefanotungamira pakuumbwa kwezvimwe zvinobva kune galactose.Mapoka eacetyl anogona kubviswa zvakasarudzika kuti awane akasiyana galactose anobva kune chaiwo mapoka anoshanda.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium munyu CAS:129541-41-9
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium munyu ikemikari inowanzoshandiswa murabhoritari tsvakurudzo uye diagnostics.Inowanzo kunzi X-Gluc uye inoshandiswa zvakanyanya se substrate yekuona beta-glucuronidase enzyme basa.
Kana beta-glucuronidase iripo, inocheka chisungo cheglucuronide muX-Gluc, zvichiita kuti kusunungurwa kwedhayi yebhuruu inonzi 5-bromo-4-chloro-3-indolyl.Kuita uku kunowanzo shandiswa kuona kana spectrophotometrically kuona kutaura kwebeta-glucuronidase enzyme mumaseru kana matishu.
Iyo sodium munyu fomu yeX-Gluc inovandudza kunyungudika kwayo mune aqueous mhinduro, kufambisa kushandiswa kwayo mumarabhoritari assays.X-Gluc inonyanya kushandiswa mukutsvagisa mamorekuru biology kudzidza gene kutaura, kusimudzira chiitiko, uye mutori wenhau gene assays.Inogona zvakare kushandiswa kuona kuvepo kwe beta-glucuronidase-inogadzira zvipenyu, senge mamwe mabhakitiriya, muzvidzidzo zvemicrobiological.
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4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9
4-Nitrophenyl-beta-D-xylopyranoside is a chromogenic substrate inoshandiswa mu enzymatic assays kuona uye kuyera basa rema enzymes anonzi beta-xylosidases.
