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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine munyu CAS:6578-06-9
BCIP-Toluidine, inozivikanwawo semunyu we5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, ikemikari inoshandiswa zvakanyanya muongororo ye molecular biology uye biochemistry. Inonyanya kushandiswa se substrate mu enzyme-linked immunosorbent assays (ELISAs) kana immunohistochemical staining techniques kuti ione basa realkaline phosphatase.
Kana BCIP-Toluidine ikachekwa nealkaline phosphatase, inochinja ruvara, zvichiita kuti pave neblue-purple precipitate. Izvi zvinoita kuti pave nekuona uye kuyera mashandiro ealkaline phosphatase mumasampuli. Kusimba kweruvara kunogona kuyerwa ne spectrophotometric uye kunoenderana zvakananga nebasa re enzyme.
Kushandiswa kweBCIP-Toluidine sechikamu chemuviri kunonyanya kushandiswa mukushandiswa kweDNA kana RNA labeling, senge in situ hybridization (ISH) kana Southern and Northern blotting. Inopa nzira yakavimbika uye inonzwisisika yekuona kuvapo kwe target nucleic acids musampuli.
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3-Aminophthalhydrazide CAS:521-31-3
3-Aminophthalhydrazide ikemikari ine fomura yemolecular C8H8N2O. Inonzi aminohydrazide derivative yephthalic acid. Iyi kemikari ine musana wephthalic acid ine maatomu maviri ehydrogen akatsiviwa neboka reamino neboka rehydrazine.
3-Aminophthalhydrazide ine mashandisirwo akasiyana-siyana mumunda wemakemikari echisikigo uye mushonga. Inogona kushandiswa senzira yekutanga mukugadzirwa kwemishonga yakasiyana-siyana uye madhayi. Pamusoro pezvo, inowanzo shandiswa mukugadzira mabhesi eSchiff, ayo ari mapakati akakosha mukugadzirwa kwemakemikari.
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4-Aminoantipyrine CAS: 83-07-8
4-Aminoantipyrine, inozivikanwawo seaminoantipyrine kana aminopyrine, imusanganiswa weorganic. Inowanzoshandiswa se reagent mukuongorora makemikari uye indasitiri yemishonga. Maumbirwo ayo emakemikari ane mhete inonhuhwirira ine boka reamino (NH2) neboka repyrazolone, zvichiita kuti ive musanganiswa unoshanda zvakasiyana-siyana.
Mukuongorora kemesitiri, 4-Aminoantipyrine inoshandiswa zvakanyanya se chromogenic reagent mu colorimetric assays. Inoshanda nezvimwe zvinhu, zvakaita se phenols ne peroxides, kuti igadzire mavara anogona kuyerwa spectrophotometrically. Hunhu uhwu hunoita kuti ibatsire mu assays dzakasiyana-siyana, kusanganisira kuona ma enzymes akadai se peroxidase, glucose, uye amylase.
Muindasitiri yemishonga, 4-Aminoantipyrine yagara ichishandiswa semushonga unonyaradza marwadzo uye unorwisa marwadzo. .
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TAPS-NA CAS:91000-53-2 Mutengo wemugadziri
N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid sodium salt, inozivikanwawo seTAPS-Na, ikemikari inoshandiswa zvakanyanya senzira yekudzivirira matambudziko mukushandiswa kwakasiyana-siyana kwehupenyu nemakemikari. Ikemikari yezwitterionic, zvichireva kuti ine zvese zviri zviviri positive ne negative charges, izvo zvinobatsira kuchengetedza pH yakagadzikana mumhinduro.
TAPS-Na ine pH iri pakati pe7.7 ne9.1, zvichiita kuti ikwanise kushandiswa inoda pH ine alkaline zvishoma. Inowanzoshandiswa mukutsvaga mapuroteni nema enzyme, pamwe chete nemaitiro e molecular biology akadai se gel electrophoresis uye DNA sequencing.
Semunyu une sodium, TAPS-Na inonyungudika zvakanyanya mumvura, izvo zvinoita kuti zvive nyore kugadzira mishonga ine buffer. Inozivikanwa nekushaikwa kwayo kwechepfu uye kusapindirana kwayo nemamorekuru ezvehupenyu, zvichiita kuti ive sarudzo inofarirwa pakuedza kwakawanda.
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5,5-Dithiobis (2-nitrobenzoic acid) CAS:69-78-3
5,5-Dithiobis (2-nitrobenzoic acid), inozivikanwawo seDTNB kana kuti Ellman's reagent, ikemikari inoshandiswa zvakanyanya muongororo yemakemikari nemishonga. Ihupfu hweyero, hwakaita sekristalo hunonyungudika zvakanyanya mumvura uye mune zvinonyungudutsa zvakasikwa.
DTNB inonyanya kushandiswa kuyera huwandu hwemapoka e-sulfhydryl (-SH) mumapuroteni nemapeptide. Musanganiswa uyu unoshanda pamwe chete nemathiols, zvichikonzera kuumbwa kwemusanganiswa we disulfide uye kuburitsa 2-nitro-5-thiobenzoate, ine ruvara rweyero runogona kuyerwa ne spectrophotometric. Nekuyera kusimba kweruvara rweyero, zvinokwanisika kufungidzira huwandu hwemathiols mumuenzaniso. Iyi nzira inowanzoshandiswa kudzidza kudyidzana kwemapuroteni nemapuroteni, kupeta mapuroteni, uye enzyme reactions dzinosanganisira mapoka ethiol.
Pamusoro pekuyera thiol, DTNB yakashandiswawo mune zvimwe zvinoshandiswa, zvakaita sekuongorora mamiriro emasero e redox, kudzidza kugadzikana kwemakemikari esimbi nesarufa, uye kuyera mashandiro ema enzyme akasiyana-siyana.
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PHENOLPHTHALEIN MONOPHOSPHATE BIS(CYCLOHEXYLAMMONIUM) MUNYU CAS:14815-59-9
Phenolphthalein monophosphate dicyclohexylammonium munyu ikemikari inotorwa kubva kuphenolphthalein, chiratidzo chepH chinowanzo shandiswa munzvimbo dzerabhoritari. Munyu we dicyclohexylammonium unoshandiswa senzira yekuongorora muongororo ye biochemical ne molecular biology. Unonyanya kushandiswa mukuyedza kana kuyedza kuona nekuyera kuvapo kwema enzymes, kusanganisira ayo anobatanidzwa muATP (adenosine triphosphate) hydrolysis kana phosphatase activity. Hunhu hwayo hwakasiyana hunoita kuti ive chishandiso chinobatsira mukuferefeta kwesainzi kwakabatana ne enzyme kinetics uye maitiro emasero..
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NSP-SA-NHS CAS:199293-83-9 Mutengo wemugadziri
NSP-SA-NHS chinhu chinosanganisira N-succinimidyl-6-(3′-pyridyldithio)hexanoate (NSP) uye N-hydroxysuccinimide (NHS). Inowanzoshandiswa semushonga unobatanidza muviri mukuita kwekubatana kwemasero, kunyanya pakubatanidza mapeptide, mapuroteni, kana mamwe mamorekuru ane amine kune mamwe mamorekuru. Chikamu cheNSP chinogonesa kuumbwa kwechisungo che thioester chakasimba nemorekuru inotarirwa, nepo chikamu cheNHS chichibvumira kuita zvinotevera neamines dzekutanga. NSP-SA-NHS inoshandiswa zvakanyanya mumabasa akasiyana-siyana ekutsvagisa uye ekurapa, kusanganisira kugadzirira immunoassays, antibody labeling, protein-protein interactions studies, uye protein immobilization.
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N-(1-Naphthyl)-3-aminopropanesulfonic Acid Sodium Munyu CAS:104484-71-1
Chiratidzo chepH: Chinhu ichi chinoratidza shanduko dzeruvara dzinoenderana nepH, zvichiita kuti chive chiratidzo chepH chinobatsira mukuyedza kwakasiyana-siyana. Chinogona kushandiswa kutarisa shanduko dzepH muzviyero zvebhayoloji, maitiro emakemikari, nedzimwe nzira.
Chiyereso cheFluorescent: N-(1-Naphthyl)-3-aminopropanesulfonic acid sodium salt ine hunhu hwe fluorescence, zvichiita kuti ikodzere zvidzidzo zve fluorescence uye mashandisirwo ekunyora mazita. Inogona kuiswa muhunyanzvi hwekuongorora nekuona uye kutevedzera mamorekuru ebhayoloji kana maumbirwo emasero.
Kuyedzwa kweEnzyme: Iyi kemikari inowanzoshandiswa se substrate kana inhibitor mukuyedzwa kwe enzyme, kunyanya iyo inosanganisira mapoka e sulfonate kana basa re enzyme rinokanganiswa nekuchinja kwe pH. Inogona kubatsira vaongorori kudzidza kinetics ye enzyme, nzira dzekudzivirira, uye kudyidzana kwe enzyme-substrate.
Chikamu cheBuffer: N-(1-Naphthyl)-3-aminopropanesulfonic acid sodium salt inogona kushandiswa sechikamu chemasisitimu chaiwo ebuffer, kunyanya ayo anoda boka resulfonic acid kana mamwe ma pH ranges. Inobatsira kuchengetedza mamiriro epH akakodzera mukuyedza.
Kugadzirwa kwemakemikari: Nekuda kwechimiro chayo chakasiyana chemakemikari, munyu weN-(1-Naphthyl)-3-aminopropanesulfonic acid sodium unogona kushanda sechivako kana chinhu chekutanga chekugadzira mamorekuru akasiyana-siyana eorganic kana mishonga.
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N-(2-Acetamide)iminodiacetic acid CAS:26239-55-4
N-(2-Acetamido)iminodiacetic acid imusanganiswa uri muboka remaamino acids anozivikanwa se chelating agents. Inowanzoshandiswa sechinhu chinosanganisa zvinhu, kunyanya mukurapa uye mumaindasitiri. Musanganiswa uyu une hunhu hwakanaka hwe metal chelating, zvichireva kuti unogona kugadzira ma complexes akagadzikana ane ma ions akasiyana-siyana e metal nekubatanidza ma complexes kuburikidza nemapoka ayo anoshanda..
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Disodium 4-nitrophenylphosphate CAS:4264-83-9
Disodium 4-nitrophenylphosphate ikemikari ine fomura yemorekuru C6H4NO6PNa2. Ihupfu hwakatsetseka hwekristaro hunoshandiswa zvakanyanya mukutsvagisa kwezvipenyu uye kushandiswa kwemolecular biology. Inowanzoshandiswa se substrate mukuyedza ma enzyme, kunyanya ma enzyme e phosphatase. Iyi kemikari inozivikanwa nekupfuura ne hydrolysis mumvura, zvichikonzera kuburitswa kwe free phosphate ion.
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6-methoxyquinoline CAS: 5263-87-6
6-Methoxyquinoline chinhu chinobva mumakemikari chine fomura yemorekuru C10H9NO. Chinhu chinonhuhwirira cheheterocyclic chine ring yequinoline yakabatana neboka remethoxy panzvimbo ye6.
Musanganiswa uyu une mashandisirwo akasiyana-siyana mukugadzirwa kwemakemikari emumuviri uye makemikari ekurapa. Unogona kushanda sechivako chekugadzirwa kwezvimwe zvinhu zvinobva muquinoline nezvimwe zvine chekuita nazvo.
Mukurapa kemikari, 6-Methoxyquinoline yakaratidza kushanda kwayo mumishonga. Yakadzidzwa nezvehunhu hwayo hwekurwisa utachiona, kurwisa malaria, uye kurwisa kenza..
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4-Benzylamino-7-nitrobenz-2-oxa-1,3-diazole CAS:18378-20-6
4-Benzylamino-7-nitrobenz-2-oxa-1,3-diazole ikemikari ine fomura yemolecular C14H11N5O3. Ikemikari ine heterocyclic ine ring ye diazole yakabatana ne ring ye benzene. Kemikari iyi inozivikanwa nehunyanzvi hwayo hwe fluorescent uye inowanzoshandiswa se dye ye fluorescent mukutsvaga kwe biological uye mashandisirwo emifananidzo. Boka re benzylamino rakabatana ne diazole ring rinowedzera kunyungudika kwayo uye kugadzikana kwayo. Boka re nitro ne oxa atomu (oxygen) iri mu diazole ring zvinopa hunhu hwayo hwemakemikari nemuviri hwakasiyana.
