4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Mhedzisiro ye4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) ndeyekushanda se substrate ye enzyme beta-glucosidase.Iyi enzyme inocheka chisungo cheglucosidic cheMUG, zvichiita kuti kusunungurwa kwe4-Methylumbliferone (4-MU) .Kushandiswa kweMUG kunonyanya kushandiswa mumunda we microbiology, kunyanya pakuona uye kuziva mabhakitiriya anogadzira beta-glucosidase.MUG inowanzoshandiswa pakuona Escherichia coli (E. coli) mumvura uye zvokudya zvekudya.E. coli ine enzyme beta-glucosidase, iyo inogona hydrolyze MUG uye kubudisa chiratidzo chefluorescent muhupo hwe ultraviolet (UV) chiedza. Iyo fluorescent pfuma ye4-MU inobvumira kuonekwa nyore uye quantification.Kana iyo MUG substrate iine hydrolyzed, iyo 4-MU inogadzirwa inoburitsa yebhuruu fluorescence, kufambisa kuzivikanwa kwebhakitiriya ine beta-glucosidase chiitiko.Iyi nzira inowanzoshandiswa mukuongorora kwezvakatipoteredza uye kuongorora kwekuchengetedza kwekudya, sezvo inopa nzira inokurumidza uye inonzwisisika yekuona kusvibiswa kwebhakitiriya.Pamusoro pekushandiswa kwayo mu microbiology, MUG inogona kushandiswawo kudzidza basa uye inhibition ye beta-glucosidase mu. biochemistry uye molecular biology research.Fluorescence yaro inogonesa kuyerwa kwe enzyme kinetics uye inogona kushandiswa kuvharidzira kune zvinogona inhibitors kana activator ye beta-glucosidase basa.Pakasiyana-siyana, MUG ikemikari yakasiyana-siyana inowana kushandiswa kwakapararira muminda ye microbiology, biochemistry, uye molecular biology yekuona. ye beta-glucosidase chiitiko uye kuzivikanwa kwebhakitiriya inoburitsa enzyme iyi.