Nitrotetrazolium Blue Chloride CAS:298-83-9

Nitrotetrazolium Bhuruu Chloride (NBT) chiratidzo che redox chinowanzo shandiswa mubiological uye biochemical assays.Iyo yakachenuruka yehupfu inoshanduka bhuruu kana yaderedzwa, zvichiita kuti ibatsire pakuona kuvepo kwemamwe ma enzymes uye metabolic chiitiko.

Mhedzisiro yekutanga yeNBT kuumbwa kweblue formazan precipitate kana yaderedzwa nemamwe maenzayimu.Uku kushanduka kwemavara kunobvumira kuona kana spectrophotometric kuona kwekuita kwe enzyme.

NBT ine zvakasiyana-siyana zvekushandisa mukutsvaga nekuongorora.Heano mamwe ekutanga kushandiswa kwayo:

Enzyme chiitiko assays: NBT inogona kushandiswa kuyera chiitiko che dehydrogenases, iyo inobatanidzwa mukuita senge cellular kufema uye metabolism.Nekutarisa kudzikiswa kweNBT kuenda kuformazan, vaongorori vanogona kuongorora kuita kwema enzymes aya.

Kuongororwa kwekushanda kwemasero ekudzivirira muviri: NBT inowanzoshandiswa muNBT kuderedza bvunzo kuti iongorore kuputika kwekufema kwemasero ekudzivirira, kunyanya phagocytes.Muedzo unoyera kugona kwemaseru aya kuburitsa reactive okisijeni marudzi, ayo anogona kuderedza NBT uye kugadzira yebhuruu precipitate.

Tsvagiridzo yeMicrobiology: NBT inoshandiswa mumicrobiology kudzidza microbial metabolism uye kuongorora basa rema enzymes chaiwo.Semuenzaniso, yakashandiswa kuona bhakitiriya nitrate reductase kana mabhakitiriya anoumba formazan.

Zvidzidzo zve cell viability: Kuderedzwa kweNBT kunobvumira vaongorori kuongorora chiitiko chemetabolism uye kugona kwemaseru.Nekuyera kusimba kwechigadzirwa chebhuruu formazan, zvinokwanisika kuona huwandu hwemasero anoshanda mune yakapihwa sampu.